Figure 5
From: Effect of nitric oxide on mitochondrial activity of human synovial cells
Protein expression of apoptotic factors. The expression of pro- and anti-apoptotic proteins was studied in synoviocytes treated with SNP at 2 mM for 48 hours. Cell protein lysates were subjected to western blot analysis. α-tubulin was used to standardize the total protein charged and expressions of bcl-2 (A) mcl-1 (B) and pro-caspase 3 (C) were evaluated. D) A representative experiment is shown. The results are shown as percentage of non-treated control cells (100%). Values are expressed as the mean ± standard error of three independent experiments (*p < 0.05).