Table 1 Description of treatment conditions and semi-quantitative measurement of bone and marrow formation in scaffold constructs and pellets.
Donor | Treatment | Implanted | Bone formed | % Bone | % Marrow |
|---|---|---|---|---|---|
1 | Control in scaffold | 3 | 0/3 | 0 | 0 |
| Â | Osteogenic in scaffold | 3 | 0/3 | 0 | 0 |
|  | Chondrogenic in scaffold | 3 | 3/3 | 9 ± 3 | 8 ± 7 |
| Â | Switch 1 (switch to osteogenic culture) in scaffold | 3 | 0/3 | 0 | 0 |
2 | Control in scaffold | 3 | 0/3 | 0 | 0 |
| Â | Osteogenic in scaffold | 3 | 0/3 | 0 | 0 |
|  | Chondrogenic in scaffold | 3 | 2/3 | 13 ± 7 | 6 ± 7 |
| Â | Switch 1 (switch to osteogenic culture) in scaffold | 3 | 0/3 | 0 | 0 |
3 | Chondrogenic in scaffold | 3* | Â | Â | Â |
| Â | Switch 1 (switch to osteogenic culture) in scaffold | 3* | Â | Â | Â |
|  | Switch 2 (+β-glycerophosphate) in scaffold | 3* |  |  |  |
3 | Chondrogenic in pellet | 3 | 3/3 | 32 ± 10 | 37 ± 24 |
| Â | Switch 1 (switch to osteogenic culture) in pellet | 3 | 0/2 | 0 | 0 |
|  | Switch 2 (+β-glycerophosphate) in pellet | 3 | 1/1 | 23 | 52 |
|  | 14 weeks chondrogenic in pellet | 3 | 2/2 | 24 ± 7 | 76 ± 7 |