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Table 1 Description of treatment conditions and semi-quantitative measurement of bone and marrow formation in scaffold constructs and pellets.

From: In-vivo generation of bone via endochondral ossification by in-vitro chondrogenic priming of adult human and rat mesenchymal stem cells

Donor Treatment Implanted Bone formed % Bone % Marrow
1 Control in scaffold 3 0/3 0 0
  Osteogenic in scaffold 3 0/3 0 0
  Chondrogenic in scaffold 3 3/3 9 ± 3 8 ± 7
  Switch 1 (switch to osteogenic culture) in scaffold 3 0/3 0 0
2 Control in scaffold 3 0/3 0 0
  Osteogenic in scaffold 3 0/3 0 0
  Chondrogenic in scaffold 3 2/3 13 ± 7 6 ± 7
  Switch 1 (switch to osteogenic culture) in scaffold 3 0/3 0 0
3 Chondrogenic in scaffold 3*    
  Switch 1 (switch to osteogenic culture) in scaffold 3*    
  Switch 2 (+β-glycerophosphate) in scaffold 3*    
3 Chondrogenic in pellet 3 3/3 32 ± 10 37 ± 24
  Switch 1 (switch to osteogenic culture) in pellet 3 0/2 0 0
  Switch 2 (+β-glycerophosphate) in pellet 3 1/1 23 52
  14 weeks chondrogenic in pellet 3 2/2 24 ± 7 76 ± 7
  1. Results are average ± SD. *Donor 3 scaffolds were not analysed because of poor cell seeding as determined in pre-implantation samples by histology and RNA content.