Figure 2

Analysis of SHG signal intensity in rat Achilles tendon from collagen surrounding normal or abnormal tenocytes. (A) Three representative spectral scans from a single normal (solid line) and a single tendinosis tendon (dashed line) are shown. The SHG signal arising from the tendon proper region peaked consistently at 440 nm as expected. (B) When SHG signal intensity was plotted as a function of position along a line drawn perpendicular to the collagen fibre bundles, the fibre bundle size could be estimated with sub-micron resolution in normal tendon, but not in tendinosis tendons.