Figure 4

Effect of gene silencing on Hsp90β transcription and protein abundance. Chondrocytes were transfected with the specific Hsp90β small interfering RNA. Total RNA and cellular proteins were extracted from control and transfected cells. A) Real-time PCR assays confirm the decrease of HSP90B gene expression in the presence of the siRNA. Data are expressed relative to gene expression without siRNA (*, p < 0.0001; n = 3). B) Representative western blot that illustrates the effect of siHsp90β at the protein level. Semi-quantitative data, obtained by densitometric analysis of the blots and normalization of each lane using α-tubulin signal, are shown at the right (*, p < 0.01; n = 3).