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Figure 1 | BMC Musculoskeletal Disorders

Figure 1

From: Hsp90β inhibition modulates nitric oxide production and nitric oxide-induced apoptosis in human chondrocytes

Figure 1

Hsp90β is modulated by proinflammatory cytokines and NO donors in chondrocytes. A) and B) Indirect immunofluorescence (IFI) analyses showing: A) the IL-1β- and TNFα- dependent increase of Hsp90β protein level; B) the increase of Hsp90β (green) after cellular stimulation with NOC-12 and SNP. Cellular nuclei, stained with DAPI, display a blue colour. Scale bars correspond to 100 μm. C) Western blot analysis on whole chondrocyte extracts stimulated for 48 h. α-Tubulin was used as loading control for normalization purposes. A representative image of the blots is shown in the left, together with the semiquantitative data obtained by densitometric analysis of the blots and normalized with the tubulin signal (on the right). Data are expressed as arbitrary volume units, and represent the mean (±SE) of four independent experiments (*, p < 0.05 and **, p < 0.01 vs basal).

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