PAR-2 receptor expression in L6 myoblasts, in myofibers and in satellite cells of skeletal muscle. A) Expression of PAR-2 receptor in L6 myoblasts and skeletal muscle (EDL) by western blot (representative sample of 4 separate experiments). B) Detection of PAR-2 protein by immunohistochemistry on EDL muscle myofibers (black arrows) and blood vessel (white arrow). C) and D) Immunohistochemistry performed on two consecutive sections showed that satellite cells identified by PAX-7 staining (C) also expressed PAR-2 receptor (D). The numbered cells 1 to 6 served to the localization of satellite cell on consecutives sections. E) Negative control of an immunohistochemistry performed without primary antibody PAR-2 or PAX-7. Bar = 50 μm.