Figure 3
From: Use of iQPR-H2O for bone regeneration and its potential in the improvement of osteoporosis
Effects of iQPR-H 2 O on MC3T3-E1 cell differentiation. ALP levels were determined in cells cultured in medium prepared with iQPR-H2O or unprocessed mineral water (control) after 1, 3, 5, and 7 days, 14 and 28 days (A). Data represent means ± SD of triplicates. *P < 0.05, as determined by independent two-sample t-tests. (B) Effect of iQPR-H2O on OPN and RUNX2 mRNA expression in MC3T3-E1 cell cultured in the presence (+) or absence (-) of iQPR-H2O for 10 days. Expression of GAPDH was determined as a loading control.