Table 1 Primer sets used for quantitative PCR analyses
From: The proteasome inhibitor MG132 reduces immobilization-induced skeletal muscle atrophy in mice
Gene | NCBI Accession Number | Forward Primer | Reverse Primer | Product Size (bp) |
|---|---|---|---|---|
Hprt1 | NM_013556.2 | 5'- gcaaactttgctttccctgg -3' | 5'- acttcgagaggtccttttcacc -3' | 85 |
MuRF-1 | DQ229108 | 5'-tgcctggagatgtttaccaagc-3' | 5'-aaacgacctccagacatggaca-3' | 143 |
Atrogin-1/MAFbx | AF441120 | 5'-aaggctgttggagctgatagca-3' | 5'-cacccacatgttaatgttgccc-3' | 223 |
Il-1β | NM_008361 | 5'-gcccatcctctgtgactcat-3' | 5'-aggccacaggtattttgtcg-3' | 230 |
Il-6 | NM_031168 | 5'-agttgccttcttgggactga-3' | 5'-tccacgatttcccagagaac-3' | 159 |
TNFα | NM_013693 | 5'-ccgatgggttgtaccttgtc-3' | 5'-tggaagactcctcccaggta-3' | 217 |