Skip to main content
Figure 3 | BMC Musculoskeletal Disorders

Figure 3

From: The proteasome inhibitor MG132 reduces immobilization-induced skeletal muscle atrophy in mice

Figure 3

Immobilization-induced atrophy was prevented by MG132. (A) Graph showing the percentage of atrophy of tibialis anterior (TA) muscles from the hindlimbs of MG132- and vehicle (DMSO)-treated mice after 7 days of immobilization. The percentage of TA atrophy was calculated by comparing each immobilized TA muscle to the respective non-immobilized contralateral TA muscle such that each animal served as its own control (n = 16). MG132 significantly attenuated the loss of muscle mass due to immobilization at day 7 post-immobilization. (B) H&E-stained cross-sections of immobilized and non-immobilized contralateral TA muscles from MG132- and vehicle (DMSO)-treated mice. The results show that MG132 prevented myofiber atrophy in the TA muscles. (C) Histogram showing the percentage area of myofiber (CSA) of TA muscles from immobilized and non-immobilized contralateral TA muscles isolated from MG132- (non-immobilized contralateral, n = 536 myofibers; immobilized, n = 643 myofibers) and DMSO-treated mice (non-immobilized contralateral, n = 241 myofibers; immobilized n = 302 myofibers). MG132 maintained the fiber size of immobilized TA muscles. Data are presented as means ± SEM. For the CSA of muscle myofibers, data are expressed as means ± SEM. (*p < 0.05, **p < 0.005, ***p < 0.001 compared to control)

Back to article page