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Figure 2 | BMC Musculoskeletal Disorders

Figure 2

From: The proteasome inhibitor MG132 reduces immobilization-induced skeletal muscle atrophy in mice

Figure 2

MG132 inhibited the key pathways involved in muscle atrophy. (A) Histogram showing the body weights of MG132- and DMSO-treated mice at day 0 (n = 8) and day 7 (n = 8) of immobilization using staples. As can be seen, there was no difference in total mean body weight between the MG132- and DMSO-treated mice. (B) TNFα, IL-6, and IL-1 mRNA levels were measured by qPCR. Data show a robust expression of TNFα, IL-6, and IL-1 in the immobilized hindlimb compared to the non-immobilized contralateral hindlimb of the DMSO-treated mice. Immobilization-induced mRNA expression was totally inhibited in the MG132-treated mice (n = 8 mice/condition). (C) qPCR expression analyses showing that MG132 was able to prevent the up-regulation of MuRF-1 and Atrogin/MAFbx in immobilized hindlimbs compared to DMSO-treated mice (n = 8 mice/condition). Data are presented as means ± SEM. (*p < 0.05, **p < 0.005, ***p < 0.001 compared to control).

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