Figure 6
From: Tenascin-C induces inflammatory mediators and matrix degradation in osteoarthritic cartilage
IL-1α induced TN-C synthesis and LPS/TN-C dependent proteoglycan loss in bovine explant cultures. Bovine explants (3 discs/well) were induced with IL-1α (0, 1, 10 ng/ml) for 48 hours and A, cartilage extracts and conditioned media analyzed in the TN-C Large ELISA. The error bars indicate SD of treatments done in triplicates; B, cartilage RNA prepared and TN-C taqman analysis performed with 50 ng RNA/well. The error bars indicate SD of treatments done in triplicates; each RNA sample from treatments/wells was tested in duplicates in the Taqman assay and results averaged. * p < 0.05. C, Bovine explants (3 discs/well) were induced with 1000 ng/ml LPS, 10 μg/ml TN-C or 5 ng/ml IL-1α in the presence or absence of TAK242 (0, 0.01, 0.1, or 1 μM) for 48 hours and the conditioned media analyzed for sGAG content. * p < 0.05.