Figure 5

TN-C or LPS treatment and ADAMTS expression in primary chondrocytes. A, Bovine chondrocytes were induced with 10 μg/ml TN-C or 1000 ng/ml LPS for 48 hours with or without TAK242. TAK242 was present 2 hours prior to induction and during the induction period. B, Human chondrocytes were induced with 10 μg/ml TN-C, 1000 ng/ml LPS, or 5 ng/ml IL-1β for 48 hours. Cells were lysed, RNA prepared and taqman assays performed with 50 ng RNA/well. HK = heat killed. ADAMTS = A Disintegrin And Metalloproteinase with Thrombospondin Motifs. The error bars indicate SD of treatments done in triplicates, each RNA sample from treatments/wells was tested in duplicates in the Taqman assay and results averaged. * p < 0.05.