Figure 1

TN-C expression in OA vs. non-OA human cartilage. A, mRNA expression of TN-C in human cartilage expressed as Taqman units normalized to GAPDH. Non-OA has been set to 1. n = 7 donors each group; error bars indicate SD; p = 0.02. B, Protein expression of TN-C in cartilage as measured in the TN-C Large ELISA. N = 7 donors each group; error bars indicate SD; p = 0.0006. C, Representative OA and non-OA cartilage extracts (5 μg protein) separated on a 3-8% tris-acetate gel, transferred, and probed with anti-TN-C (EGF-like domain) antibody, 4F10TT that detects all forms of TN-C. Lanes are O = OA; N = Non-OA; S = standard human TN-C protein (Millipore). Bands are marked as L = large variant and S = small variant.