The oncofetal gene survivin is re-expressed in osteoarthritis and is required for chondrocyte proliferation in vitro

Table 1 Details of the antibodies used

Method	Detected	Primary antibody	(μg/ml)	secondary antibody	(μg/ml)
	protein
IB	Survivin	pAB AF886 (R&D Systems)	1,0	Polyclonal immunoglobulins/HRP-conjugated (DAKO)	0,3
IB	Survivin	pAB 500.201 (Novus Biologicals)	1,0	Polyclonal immunoglobulins/HRP-conjugated (DAKO)	0,5
IF	Survivin	pAB AF886 (R&D Systems)	10,0	Red fluorescent dye-labeled immunoglobulin (Invitrogen)	10,0
IF	Survivin	pAB 500.201 (Novus Biologicals)	10,0	Red fluorescent dye-labeled immunoglobulin (Invitrogen)	8,0
IF	Survivin	mAB clone 60.11 (Novus Biologicals)	6,0	Red fluorescent dye-labeled immunoglobulin (Invitrogen)	8,0

The primary and secondary antibodies used for the immunofluorescence and immunoblotting are listed, with inclusion of the sources, purposes and concentrations. IB: immunoblotting; IF: immunofluorescence.

ISSN: 1471-2474