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Table 1 Details of the antibodies used

From: The oncofetal gene survivin is re-expressed in osteoarthritis and is required for chondrocyte proliferation in vitro

Method Detected Primary antibody (μg/ml) secondary antibody (μg/ml)
  protein     
IB Survivin pAB AF886 (R&D Systems) 1,0 Polyclonal immunoglobulins/HRP-conjugated (DAKO) 0,3
IB Survivin pAB 500.201 (Novus Biologicals) 1,0 Polyclonal immunoglobulins/HRP-conjugated (DAKO) 0,5
IF Survivin pAB AF886 (R&D Systems) 10,0 Red fluorescent dye-labeled immunoglobulin (Invitrogen) 10,0
IF Survivin pAB 500.201 (Novus Biologicals) 10,0 Red fluorescent dye-labeled immunoglobulin (Invitrogen) 8,0
IF Survivin mAB clone 60.11 (Novus Biologicals) 6,0 Red fluorescent dye-labeled immunoglobulin (Invitrogen) 8,0
  1. The primary and secondary antibodies used for the immunofluorescence and immunoblotting are listed, with inclusion of the sources, purposes and concentrations. IB: immunoblotting; IF: immunofluorescence.
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BMC Musculoskeletal Disorders

ISSN: 1471-2474

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