Figure 4

Functional parameters after survivin knockdown in primary human chondrocytes. (A) Cell cycle distribution at 48 hours after survivin knockdown (means ± SEM) as measured by the FACS/PI-staining method. GFP was used as a negative control. The original results of one representative experiment are shown. (B) BrdU uptake measured in relative light units at 48 hours after knockdown of survivin (means ± SEM). (C) Caspase activity at 24 hours after survivin knockdown in cells grown under regular culture conditions (unstressed) and under in vitro ischemia (1% oxygen, glucose deprivation) (means ± SEM). The results shown are a representative experiment of three independent experiments each performed in triplicate.