Survivin expression in human paraffin-embedded osteoarthritic cartilage. (A-F) Immunofluorescence for survivin (red) in macroscopically and microscopically arthritic cartilage (A, C, E) and adult non/moderate arthritic cartilage (paraffin-embedded tissue sections) (B, D, F). Chondrocytes were stained with 4,6-diamidino-2-phenylindole (blue) in the identical positions. Original magnifications: 200 × (A-D) and 400 × (E, F). (G) Cartilage from 10 patients was analyzed for the mRNA expression of survivin by semiquantitative real-time PCR. Histopathologic non/moderate arthritic cartilage samples (N) and osteoarthritic degenerated cartilage (OA) were analyzed by quantitative PCR. The relative gene expression rates of three representative patients are shown.