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Table 1 real-time PCR investigation.

From: Characterization of the age-dependent intervertebral disc changes in rabbit by correlation between MRI, histology and gene expression

Gene (Abbreviation)

Accession number (Gene Bank)

Forward primer (5'-3')

Reverse primer (5'-3')

Product length

GAPDH

NM_002046

agccacatcgctcagaca

gcccaatacgaccaaatcc

66 pb

Type II collagen

COL2A1

D83228

acagcaggttcacctataccg

cccacttaccggtgtgtttc

60 pb

Aggrecan

AGC1

L38480

gaggatggcttccaccagt

tggggtacctgacagtctga

61 pb

Type I colagen

COL1A1

D49399

agcgatggtcctccaggt

gccagggtaaccacgttct

63 pb

Matrix metalloproteinase 13

MMP13

NM_001082037

ttttgaagacacgggcaag

tcatcatagctccagacttggtt

60 pb

Bone Morphogenic Protein 2

BMP2

NM_001082650

tgcagaacttcaggtttttcg

tggaaaccgctgtcgtct

63 pb

Matrix Gla protein

MGP

D21265

tggatataatgctgcttacaatcg

tttccaatcttattcagctctgc

64 pb

P21-activated protein kinase I

P21

NM_001082756

agaaagaaaaagaacgaccagaga

cgtggatggtgtgctcaa

60 pb

  1. GenBank reference of the genes evaluated and oligonucleotide primers used for real-time PCR.