Figure 3

The effect of ZA on LM8 cell apoptosis. The LM8 osteosarcoma cells were cultured on type I collagen gel and treated with the vehicle control (A) or 5 μmol/L ZA (B) for 24 hours (X400). Apoptotic cells were stained using the TUNEL assay (brown). The non-apoptotic cells were stained blue. Alternatively, the LM8 cells treated with the vehicle control (C), 1 μmol/L ZA (D), or 5 μmol/L ZA (E) for 24 hours were analyzed by flow cytometry after staining with Annexin V-FITC/PI. The table shows the mean ± SE from n = 3 independent experiments, where each quadrant represents the percentage of cells in early apoptosis (Lower right, LR), late apoptosis (Upper right, UR), necrosis (Upper left, UL), and healthy cells (Lower left, LL).