Figure 1

Increased expression of muscle miRNAs in the skeletal muscle of mdx mice. (A) Fold change of the expression of indicated miRNAs in the skeletal muscle of 1 month old mdx mice (n = 3) as detected by qRT-PCR analyses. Statistical differences were determined using the Student's t-test; (*) P < 0.05. (B) Northern blot analyses of the expression of miR-1 and miR-206 in the skeletal muscle of mdx and the wild type control mice (n = 3). Precursors and mature miRNAs are indicated. tRNAs were used as a loading control. (C) The intensity of mature miRNA products from Northern blot was quantified using the Photoshop Histogram Analysis and was plotted using Microsoft excel and is shown as relative expression level after normalized by tRNA. Statistical differences were determined using the Student's t-test; (*) P < 0.05.