Figure 2

Forskolin effectively reduced TGF-β ₁ stimulation of α-SMA, FN1-EDA, CTGF, COL1A2 and COL3A1. Fibroblast cultures from CT-, PF- and DC- tissues were left untreated or were stimulated with forskolin (10 μM) in the presence or absence of TGF-β₁ (2 ng/ml). Twenty-four hours later, mRNA expression levels of α-SMA (a), FN1-EDA (b), CTGF (c), COL1A2 (d) and COL3A1 (e) were analyzed by real time RT-PCR. In each experiment at least three independent cultures obtained from all the three cell types were used. Values are means ± SEM of six independent studies performed in duplicate. Statistical analyses were performed using two-way ANOVA.