Figure 1From: Reversal of TGF-β1 stimulation of α-smooth muscle actin and extracellular matrix components by cyclic AMP in Dupuytren's - derived fibroblasts Basal mRNA expression of ECM proteins was significantly elevated in DC-derived fibroblasts. Real time RT-PCR was performed on RNA extracted from 6 independent primary cultures derived from CT-, PF- and DC- tissues to determine the mRNA expression levels of COL1A2 (a), COL3A1 (b), FN1-EDA (c), and CTGF (d). Values are means ± SEM of three independent experiments performed in duplicate. Statistical analyses were performed using two-way ANOVA.Back to article page