Table 1 Location of primerpairs in the coding region of the human calcitonin receptor.
From: Investigation of the direct effects of salmon calcitonin on human osteoarthritic chondrocytes
Gene | Forward | Reverse | Product length | Product Tm |
|---|---|---|---|---|
SURF1 | 5-CAGCAGACCCAATGGAAC-3 | 5-CCAGGATGGTGACTCCC-3 | 238 bp | ~83.6 |
Coll2A | 5-AGGATGGTGACTCCCAAG-3 | 5-GTCCATGGGTGCAATGTCAA-3 | 136 bp | ~83.5 |
CalcR 1 | 5-GACAACTGCTGGCTGAGTG-3 | 5-GAAGCAGTAGATGGTCGCAA-3 | 321 bp | ~84.1 |
CalcR 2 | 5-TGATTCATTTCCAGGGCTTC-3 | 5-GCTGGTTCATTCCTCAGCTC-3 | 220 bp | ~88.2 |
CalcR 3 | 5-GGTAACCCTGCACAAGAAC-3 | 5-AAGCGTTGCTTCTCAGTAAA-3 | 235 bp | ~82.0 |
CalcR 4 | 5-ACACCGGCTGGAGTATTGTC-3 | 5-AAAATCCCCAGGGAAATCAC-3 | 257 bp | ~79.8 |