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Table 1 Location of primerpairs in the coding region of the human calcitonin receptor.

From: Investigation of the direct effects of salmon calcitonin on human osteoarthritic chondrocytes

Gene Forward Reverse Product length Product Tm
SURF1 5-CAGCAGACCCAATGGAAC-3 5-CCAGGATGGTGACTCCC-3 238 bp ~83.6
Coll2A 5-AGGATGGTGACTCCCAAG-3 5-GTCCATGGGTGCAATGTCAA-3 136 bp ~83.5
CalcR 1 5-GACAACTGCTGGCTGAGTG-3 5-GAAGCAGTAGATGGTCGCAA-3 321 bp ~84.1
CalcR 2 5-TGATTCATTTCCAGGGCTTC-3 5-GCTGGTTCATTCCTCAGCTC-3 220 bp ~88.2
CalcR 3 5-GGTAACCCTGCACAAGAAC-3 5-AAGCGTTGCTTCTCAGTAAA-3 235 bp ~82.0
CalcR 4 5-ACACCGGCTGGAGTATTGTC-3 5-AAAATCCCCAGGGAAATCAC-3 257 bp ~79.8
  1. Housekeeping gene: SURF1; Collagen type II: Coll2A; Calcitonin receptor 1-4: CalcR 1-4.
  2. Length of PCR products, base pairs (bp) and melting temperature (T m )