Figure 8
VEGF, IL-8 and MMP-9 production measured in LPS-stimulated cell supernatants of RA SF macrophages under normoxia and hypoxia. Cells were stimulated with LPS for 48 hours and pretreated with inhibitors (10 μM) of ERK, PI3K, and CAMKII pathways in a normal incubator (black bars) or in a hypoxia incubator (1% O2, Striped bars). PD = PD98059-MEK inhibitor, LY = LY294002-PI3K inhibitor, KN = KN93-CaMKII inhibitor, SM = SMP114-CaMKII inhibitor. Significance of inhibition was calculated compared to LPS stimulated sample with one-way ANOVA with Dunnett's post test: * p < 0.05, ** p < 0.01.