Protein production in cell supernatants of RA SF macrophages. Cells were stimulated with LPS and pretreated with different concentrations of inhibitors of ERK, PI3K, and CAMKII pathways. Protein production was measured in cell supernatants by ELISA. (A) VEGF production by SF macrophages (SFM) (n = 6). PD = PD98059-MEK inhibitor, LY = LY294002-PI3K inhibitor, KN = KN93-CaMKII inhibitor, SM = SMP114-CaMKII inhibitor. Significance of inhibition was calculated compared to LPS stimulated sample with one-way ANOVA with Dunnett's post test: * p < 0.05, ** p < 0.01.