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Figure 5 | BMC Musculoskeletal Disorders

Figure 5

From: Screening of protein kinase inhibitors identifies PKC inhibitors as inhibitors of osteoclastic acid secretion and bone resorption

Figure 5

Detection of PKC in both osteoclast membranes and osteoclast lysate. CD14+ monocytes were cultured in the presence of M-CSF and RANKL for 12 days to mature osteoclasts were present. The osteoclasts were either used to make a lysate of mature human osteoclasts using RIPA+++, or they were used for microsome isolation. The expression of PKC, together with V-ATPase B2 as a positive control, was analyzed by loading 10 μg of lysates and microsomes on SDS-PAGE gels with subsequent immunoblotting.

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BMC Musculoskeletal Disorders

ISSN: 1471-2474

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