Figure 3

Hysteresis with standard errors (shown at alternating loading rates for each sample) for the means of the loading rates for control samples at 4°C (identified by open circles) and samples frozen to -20°C (identified by open triangles), -80°C (identified by open squares), or snap frozen in liquid nitrogen and stored at -80°C (identified by crosses). Plot A identifies the hysteresis of samples immediately after thawing and plot B identifies the hysteresis after 4 hours storage at 22°C. This figure shows increased levels of viscous flow over faster loading rate frequencies for samples snap frozen in liquid nitrogen and stored at -80°C (A and B) and those frozen at -20°C (B). The small dotted line in plot B represents the 0 hour control data.