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Figure 8 | BMC Musculoskeletal Disorders

Figure 8

From: Silencing Dkk1 expression rescues dexamethasone-induced suppression of primary human osteoblast differentiation

Figure 8

This increase in β-catenin expression by silencing Dkk1 expression results in an increase in Wnt/β-catenin signaling through TCF/LEF mediated transcription. Primary human osteoblasts were transiently transfected with pBAR (wild type promoter) and pfuBAR (mutant promoter), and subsequently treated with siRNA targeting Dkk1 expression or scrambled control RNA. A significant increase in luciferase activity was observed in the siRNA transfected cells when compared to scrambled control cells in the pBAR reporter cells, whilst luciferase activity in the pfuBAR cells remained unchanged (c). ***p < 0.001 versus scrambled control.

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