Figure 7
Immunoflourescence microscopy demonstrating β-catenin expression in primary human osteoblasts transfected with scrambled control RNA (a) or siRNA targeting Dkk1 expression (b). siRNA transfected primary human osteoblasts demonstrated a strong perinuclear and intranuclear staining of β-catenin, in comparison to scrambled control primary human osteoblasts. This represents activation of Wnt/β-catenin signaling in response to silencing Dkk1 expression.