Figure 3

Adipogenic potential of human synovial or bone marrow MSCs cultured with autologous human serum or FBS. Synovial MSCs and bone marrow MSCs precultured with autologous human serum or FBS at passage 0 were replated at 100 cells in 60-cm² dishes. These cells were cultured in the medium containing the same serum used at passage 0 for 14 days to form cell colonies. Then, the medium was switched into adipogenic medium containing the same serum used at passage 1 and cultured for an additional 21 days. The dishes were stained with Oil Red-O for adipogenesis. (A) Adipocyte colonies stained with Oil Red-O. Bar 5 cm. (B) Total cell colonies. The same dishes shown in (A) were stained with Crystal Violet. (C) Oil Red-O positive colony forming efficiency (%). The ratio of Oil Red-O positive colony number to the total colony number was calculated in synovial and bone marrow MSCs (n = 3). Average value with standard deviation from 3 donors is shown respectively. * = P < 0.01; Oil Red-O positive colony forming efficiency in the same donor with and without adipocyte induction. (D) Adipocyte-related mRNA expressions by reverse transcription-PCR analysis. Synovial or bone marrow MSCs at passage 0 were replated at 5000 cells/cm² and cultured in the absence or presence of calcification medium with autologous human serum or FBS for 21 days. PPARγ indicates peroxisome proliferator activated receptor γ; and FABP4, fatty acid binding protein 4.