Figure 1

Growth unaffected in HU mice, and changes in bone histomorphometry. A- Mice weight (mean +/- SEM). The values are obtained from the measure of the weight of 6 mice per point for each condition, below. Means +/- SEM. One-way ANOVA shows no significant difference for p < 0.05. B- Photomicrographs of Hematoxylin-Eosin. Stained sections from tibiae of mice at different days in HU were taken at the growth plate, cortical bone at mid-diaphysis, bone marrow in the distal region of the tibia. Enlargement is 200×. On the bottom the whole of the proximal epiphysis, 40×. C- Bone morphometric parameters by μCT, in control and HU14 mice. Apparent volume density (AVD), trabecular bone volume density (BV/TV trabecular), trabecular thickness (Tb.Th), trabecular number (Tb.N), connectivity density (Conn.D), diaphyseal diameter (Dia.Dia), cortical thickness (Cort.Th), medullary cavity diameter (Med.Dia). Data are mean ± SEM obtained in 6 mice per condition. *, p < 0.05. Black columns control, white columns HU14. D- 3D μCT femoral images from control and HU for 14 days.