Quantification of captured signal in minor salivary glands in anti-centromere antibody (ACA) + and ACA- patients with Sjögren's syndrome (SS). The captured green signal from 14 ACA+ and 48 ACA- SS patients was calculated by WinROOF software under certain conditions. The figures represent actual measurements in units of μm2 under a fixed magnification ratio of the optical microscope (×200). A scatter plot of the processed data is shown. Quantification results from the patients with and without ACA showed 65052.2 ± 14520.6 μm2 and 26251.3 ± 14249.8 μm2 with significance (p = 1.3 × 10-12). Student's t test was used for statistical evaluation. A P value less than 0.05 was considered statistically significant.