Azan Mallory staining of anti-centromere antibody (ACA) + and ACA- patients with Sjögren's syndrome (SS). To detect the existence of collagenous fiber in minor salivary glands in SS patients, Azan Mallory staining was performed for all patients. The lower panels show Azan Mallory staining results of ACA+ and ACA- SS patients. To clarify the amount of collagenous fiber, the image analyzer WinROOF was employed. The green signal in the upper panels shows the captured form of blue staining produced by Azan Mallory staining. (Original magnification × 200).