Figure 1

Expression of TRPM3 in FLSs of synovium in rheumatoid arthritis. (a) PCR products from analysis of mRNA isolated from primary cultured FLSs from patients with (+) and without (-) prior reaction with reverse transcriptase (RT). On the left is the DNA marker ladder (M). The expected TRPM3 mRNA product size is indicated with an arrow. (b) Serial sections of RA synovial biopsy labeled with the primary (1°) anti-TRPM3 antiserum present (left image) or absent (right image). Positive labeling is green. The scale bar is 50 μm. (c) A section of RA synovial biopsy labeled with anti-TRPM3 antiserum (brown) and counter stained with haematoxylin to label cell nuclei (purple). The lining of the synovium (i) and a blood vessel (ii) are indicated by arrows.