Evaluation of MMP generated resorption and MMP secretion. Mature human osteoclasts were seeded on bone slices and cultured for 5 days in the presence or absence of the indicated inhibitors. A) ICTP levels were measured in culture supernatants. B) Gelatinase zymografi of pooled culture supernatants. MMP-9 is used as marker. A representative gel is shown. Based on analysis of three individual gels in ImageJ, relative band intensities, reflecting MMP-9 activity, were calculated. Relative intensities are given ± SD.