Evaluations of resorbed bone area by different types of osteoclast inhibitors. Mature human osteoclasts were seeded on bone slices and cultured for 5 days in the presence or absence of the indicated inhibitors. A) Pits were stained and pictures of the indicated conditions were taken. B) The stained pits were counted using CAST grid. Percent resorbed bone area was calculated and plotted. Statistical tests were performed by ANOVA with Dunnet's multiple comparison test against the vehicle group. Scale bars = 200 μm.