Figure 3

Radiographic analysis of the BMP-2 implantation model. This model uses 20 μg BMP-2 implanted into the hind limb to induce ectopic bone formation in 3 weeks. The effects of local SB431542 co-delivery were quantified by microCT measurement of bone volume in the entire bone pellets (A). Relative to BMP-2 alone, SB431542 did not increase bone formation (47% decrease, P < 0.06 for 92 μg SB431542; 25% decrease, P < 0.21 for 384 μg SB431542). This is further illustrated by representative microCT reconstructions of bone nodule cross-sections.