Figure 5From: Retinoblastoma binding protein-1 (RBP1) is a Runx2 coactivator and promotes osteoblastic differentiation RBP1 is a Runx2 coactivator in both U2OS osteosarcoma and mouse calvarial osteoblast cell models. Mouse calvarial osteoblast (A) or human U2OS osteosarcoma (B) cells were transiently transfected (n = 3) with either the p6OSE2-Luc reporter construct or the mouse osteocalcin luciferase reporter construct as described under "Methods." The black bars represent the mean ± standard deviation. A single asterisk (*) represents significance of p < 0.01 (ANOVA) relative to the reporter construct alone and a double asterisk (**) represents significance of p < 0.01 (ANOVA) relative to the reporter and Runx2 expression construct alone. U.D. stands for undetectable.Back to article page