Figure 3

Real-time QPCR analysis demonstrates suppressed bone marker gene expression in the CalOB-ΔRBP1 cell line. Total RNA was extracted from CalOB-Control (solid line) and CalOB-ΔRBP1 (dotted line) cells treated as in Figure 2 and processed for real-time QPCR analysis (n = 3) for the indicated genes (A-F). The data are expressed as target gene expression relative to TBP controls and normalized to the day 0 CalOB-Control cell line. The data points represent the mean ± standard deviation. The asterisks represents statistical significance at the p < 0.01 level (ANOVA).