Figure 1

Type 1 collagen culture effects on β-catenin accumulation in DD and PF-cells. A Representative western immunoblots of PF cells (PF1) and DD cells (PD1) cultured on type 1 collagen (1.9 mg/ml) for 0, 24, 48 and 72 hours prior to cell lysis and 8% PAGE, probed for β-catenin. Lysate from CaCo cells, which express high levels of β-catenin, was included as a positive control. β-actin levels were assessed by immunoblotting to confirm equal total protein loading. As indicated, (arrow) a decrease in β-catenin levels is evident in DD cells cultured on type 1 collagen for 72 hours. B Pooled densitometric analysis of triplicate analyses of the samples assessed in A normalized to β-actin. A significant decrease in band density indicating decreased β-catenin levels is evident in DD cells cultured on type 1 collagen for 72 hours relative to 0 hour cultures (*, p < 0.05).