Effect of PMMA on endogenous LXA
production. A, LXA4 content in culture media was monitored with ELISA kit. **, P < 0.01 compared to macrophages+PMMA; #, P < 0.05 compared with macrophage+OB+PMMA. B, Inhibition of 15-LO siRNA on 15-LO mRNA expression measured by RT-QPCR. Results were normalized to GAPDH and expressed as fold induction over cells co-cultured with OB without 15-LO siRNA. C, Inhibition of 15-LO siRNA on 15-LO protein expression measured by western blotting. GAPDH was applied as internal control.