Skip to main content

Table 1 Oligonucleotide sequences for quantitative PCR (qPCR)

From: Myoblast sensitivity and fibroblast insensitivity to osteogenic conversion by BMP-2 correlates with the expression of Bmpr-1a

Target cDNA sequence

Sense primer

Anti-sense primer

MyoD

5'-CGGCTACCCAAGGTGGAGAT-3'

5'-ACCTTCGATGTAGCGGATGG-3'

Bmpr-1a

5'-ACATCAGATTACTGGGAGCC-3'

5'-TATAGCAAAGCAGCTGGAG-3'

Bmpr-2

5'-GTGCCCTGGCTGCTATGG-3'

5'-TGCCGCCTCCATCATGTT-3'

Gapdh

5'-GCCACCCAGAAGACTGTGGATGGC-3'

5'-CATGTAGGCCATGAGGTCCACCAC-3'

Ap

5'-GGGACTGGTACTCGGATAACGA-3'

5'-CTGATATGCGATGTCCTTGCA-3'

Ocn

5'-CGGCCCTGAGTCTGACAAA-3'

5'-GCCGGAGTCTGTTCACTACCTT-3'

Back to article page

BMC Musculoskeletal Disorders

ISSN: 1471-2474

Contact us