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Table 1 Oligonucleotide sequences for quantitative PCR (qPCR)

From: Myoblast sensitivity and fibroblast insensitivity to osteogenic conversion by BMP-2 correlates with the expression of Bmpr-1a

Target cDNA sequence Sense primer Anti-sense primer
MyoD 5'-CGGCTACCCAAGGTGGAGAT-3' 5'-ACCTTCGATGTAGCGGATGG-3'
Bmpr-1a 5'-ACATCAGATTACTGGGAGCC-3' 5'-TATAGCAAAGCAGCTGGAG-3'
Bmpr-2 5'-GTGCCCTGGCTGCTATGG-3' 5'-TGCCGCCTCCATCATGTT-3'
Gapdh 5'-GCCACCCAGAAGACTGTGGATGGC-3' 5'-CATGTAGGCCATGAGGTCCACCAC-3'
Ap 5'-GGGACTGGTACTCGGATAACGA-3' 5'-CTGATATGCGATGTCCTTGCA-3'
Ocn 5'-CGGCCCTGAGTCTGACAAA-3' 5'-GCCGGAGTCTGTTCACTACCTT-3'