Figure 5

Forced MyoD expression in NIH/3T3 fibroblasts increases Bmpr-1a expression. NIH/3T3 cells were transduced using LV-MyoD and LV-eGFP lentiviral vectors. Fibroblasts transduced with LV-MyoD developed an elongated morphology that resembled myotubes (A). Scale bar = 50 μm. Three days after the initial transduction, cells were cultured for a further 3 days in the presence or the absence of BMP-2. Gene expression for MyoD (B), Bmpr-1a (C) and Ap (D) were measured using qPCR, relative to Gapdh. Comparative expression analysis was performed relative to non-transduced, untreated NIH/3T3 fibroblasts. The majority of values were statistically significant (*p < 0.05, **p < 0.01), however, substantive changes in gene expression (>4-fold increase) were only observed in LV-MyoD transduced cells.