Effect of 2ME2 on Qualitative Histopathology Parameters. Five mice from each group were necropsied on study day 21. At necropsy, both right and left hind paws were collected, preserved in 10% buffered formalin, and processed for histopathology and histomorphometric analyses. Qualitative histopathology scoring was performed using acid phosphatase/toluidine blue stained sections on blinded samples. The area of interest included the area from the talus-navicular joint to the navicular to the second metatarsal joint. Asterisks indicate groups statistically different from vehicle control (p < 0.05).