Maximum Antiarthritic Activity is Observed With Daily Dosing with 2ME2. Female Balb/c mice (5–7 weeks of age) were injected i.v. with a monoclonal antibody cocktail against type II collagen (2 mg/mouse). Twenty-four hours later, mice were treated i.p. with 25 μg LPS (E. coli strain 0111:B4). Subsequent to LPS administration, cohorts of mice (n = 10/group) began treatment by daily oral gavage with either vehicle control or 2ME2 at 100 mg/kg for 10 consecutive days. Additional cohorts of mice were treated weekly or twice a week with 2ME2 at 100 mg/kg. Mice were assessed on study days 2, 4, 8, and 10 for clinical severity of disease progression by observing paw swelling, erythema, and a loss of passive use. Maximum arthritic score is 16 for each mouse. Asterisks indicate groups statistically different from vehicle control on study day 10 (p < 0.05).