Table 1 Gli3 primers for DGGE
|  | Primer sequence | Annealing Temp (°C) | Product size (bp) |
|---|---|---|---|
Promoter 1 F | *AGCTTGCAGTTCCCTTGC | 60 | 684 |
R | GTCCGACAATTTCTAACATCGA | Â | Â |
Promoter 2 F | *GCCTTCGATGTTAGAAATTGTCG | 62 | 668 |
R | TGGGCTGCTGGTAATCCCTGTGC | Â | Â |
Exon 1 F | *TTTTGGAAAGTTGATGGCTCT | 60 | 229 |
R | GGCTGCTGGTAATCCCTGT | Â | Â |
Exon 2 F | *AATTGCTCCTTAAAGTAGTT | 60 | 335 |
R | CATAGCTCCTGAACAAGTG | Â | Â |
Exon 3 F | *CAATGTTGCTTTGTGAAT | 60 | 234 |
R | TAAAAGCCAGCATCTCGT | Â | Â |
Exon 4 F | *CCCCTTGTATCTGGTTTT | 57 | 316 |
R | GTCTACTTTATACACGTCCC | Â | Â |
Exon5 F | *ATTGCTGATGTGGGTTGT | 60 | 297 |
R | GTTGCCTTTGCCATTTCC | Â | Â |
Exon 6 F | *TAGGCAAGTAGCAATAAATAG | 60 | 303 |
R | ACATAATGGATTCAGGAAAA | Â | Â |
Exon 7 F | *GCTCAGCGTTTAAGTGAT | 61 | 268 |
R | GCATCGACCTGTCCCTCT | Â | Â |
Exon 8 F | *GGGATTGGAGAATTATCAG | 60 | 299 |
R | AGTCTTGGGAGGAGTGGG | Â | Â |
Exon13 F | *TTCCTTTCCACTTGACCCC | 60 | 391 |
R | AAAACCCTGAGCAGATGCA | Â | Â |
Exon 14-1 F | *TGTGAGGCAGGCAATGTG | 53 | 325 |
R | GGAGAAGCAGGGCGAGAT | Â | Â |
Exon 14-2 F | *ATCTCGCCCTGCTTCTCC | 55 | 489 |
R | GCTGCTGAGGCTGCTGAA | Â | Â |
Exon 14-3 F | *AGAGGATGAGCCTGAAGACG | 56 | 486 |
R | GCTGCTCGTACCCTGCTT | Â | Â |
Exon 14-4 F | *CTGATGCCAACCTGAACG | 56 | 479 |
R | TCCACTGGTGCCACTTCC | Â | Â |
Exon 14-5 F | *TGGTCGTCCACCCGCAGAA | 58 | 490 |
R | GGCCCTTGGTAGATGTTGATGT | Â | Â |
Exon 14-6 F | *AGATGCTTGGGCAGATTAG | 60 | 350 |
R | TGAACCAGCTTTCGTGTC | Â | Â |
Exon 14-7 F | *CGCTGTGCTCTAATCTGC | 60 | 491 |
R | TATTGATTTCCGTTGGTTG | Â | Â |