Figure 4From: Functional activation of proline-rich tyrosine kinase2 (PYK2) in peripheral blood mononuclear cells from patients with systemic lupus erythematosus Enhanced PYK2 phosphorylation in PBMCs in response to lymphocyte activation stimulated by PMA. (A) PBMCs obtained from healthy donors (n = 24), RA patients (n = 32) and SLE patients (n = 48) were incubated in 24-well culture dishes in 5% CO2 at 37°C for 24 h for with no stimulation (medium), or stimulation with PMA (1 ng/ml). Lysates prepared from 2 × 106 cells were analyzed by western blotting for the expression of p-PYK2 as indicated in patients and methods. (B) p-PYK2 expressed relative to the value obtained with PMA stimulation. Bars represent the mean and standard deviation of three separate experiments. Statistical analysis: * = p < 0.05.Back to article page