Enhanced expression and activation of PYK2 in SLE patients. (A) Representative blot of total PYK2 and p-PYK2 in PBMCs lysates proteins obtained from healthy donors (n = 24), patients with RA (n = 32), and patients with inactive (n = 12) or active (n = 36) SLE. Total proteins obtained from PBMCs lysates were examined by Western blotting analysis for PYK2 and p-PYK2 as indicated in patients and methods. Lane 1 and 2, healthy donors;Lane 3 and 4, RA patients;Lane 5 and 6, inactive SLE patients;Lane 7 and 8, active SLE patients. (B) The ratio between the optical density of specific bands and β-actin of the same sample was calculated and expressed graphically. The experiments for each sample were repeated at least three times and bars represent the mean and standard deviation of three separate experiments. Statistical comparison of all groups was made with healthy donors. The significance level was set at* = P < 0.05.