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Table 1 Quantitative Scoring of the % of Colonies Containing Type I Collagen, Type II Collagen, Chondroitin Sulfate or Keratan Sulfate*

From: Human disc cells in monolayer vs 3D culture: cell shape, division and matrix formation

  Type I Collagen Type II Collagen Chondroitin Keratan Sulfate
    Sulfate  
Passage 1 93.9 ± 3.7 86.2 ± 1.7 90.0 ± 3.8 80.9 ± 7.0
Passage 2 96.7 ± 1.0 85.1 ± 3.0 87.3 ± 2.1 83.5 ± 2.6
Passage 3 98.1 ± 0.6 88.5 ± 2.3 91.6 ± 3.9 85.1 ± 3.0
Passage 4 97.4 ± 0.5 85.2 ± 1.6 96.2 ± 1.1 87.2 ± 2.3
  1. *Data are mean ± s.e.m. for colonies scored for the presence of the specific ECM component. Data are based on a mean of 240 colonies scored on each subject's cells cultured for each passage; cells from five individuals were evaluated.