Altered mRNA expression of genes related to nerve cell activity in the fracture callus of older rats: A randomized, controlled, microarray study
© Meyer et al; licensee BioMed Central Ltd. 2004
Received: 25 March 2004
Accepted: 03 August 2004
Published: 03 August 2004
The time required for radiographic union following femoral fracture increases with age in both humans and rats for unknown reasons. Since abnormalities in fracture innervation will slow skeletal healing, we explored whether abnormal mRNA expression of genes related to nerve cell activity in the older rats was associated with the slowing of skeletal repair.
Simple, transverse, mid-shaft, femoral fractures with intramedullary rod fixation were induced in anaesthetized female Sprague-Dawley rats at 6, 26, and 52 weeks of age. At 0, 0.4, 1, 2, 4, and 6 weeks after fracture, a bony segment, one-third the length of the femur, centered on the fracture site, including the external callus, cortical bone, and marrow elements, was harvested. cRNA was prepared and hybridized to 54 Affymetrix U34A microarrays (3/age/time point).
The mRNA levels of 62 genes related to neural function were affected by fracture. Of the total, 38 genes were altered by fracture to a similar extent at the three ages. In contrast, eight neural genes showed prolonged down-regulation in the older rats compared to the more rapid return to pre-fracture levels in younger rats. Seven genes were up-regulated by fracture more in the younger rats than in the older rats, while nine genes were up-regulated more in the older rats than in the younger.
mRNA of 24 nerve-related genes responded differently to fracture in older rats compared to young rats. This differential expression may reflect altered cell function at the fracture site that may be causally related to the slowing of fracture healing with age or may be an effect of the delayed healing.
Bone formation to bridge the fracture gap following skeletal fracture slows with age in both humans [1–6] and rats [7–9]. While young, 6-week-old rats reach radiographic union by 4 weeks after femoral fracture, adult, 26-week-old rats require 10 weeks, and older, 52-week-old rats need in excess of 6 months . Despite this increased time to radiographic union with age, there was no increase in the time of expression of Indian hedgehog or any of the bone morphogenetic proteins in the fracture callus for adult rats  or for older rats [11, 12]. Radiographic union for adult and older rats occurred well after the time of expression of these skeletally active cytokines [10, 11]. Except for markers of osteoblast activity and bone matrix formation, few genes remain up-regulated during the time period when bone forms to bridge the fracture gap [10–12].
These earlier studies done with RT-PCR revealed a paucity of data for genes differentially expressed by age. We had hypothesized that bone formation to bridge the fracture gap would be under a negative-feedback control system. Thus, the genes which stimulate bone formation should be up-regulated in adult or older rats to attempt to accelerate their slower progression of bony healing. This was not observed in adult  or older [11, 12] rats. Either bone formation to bridge the fracture gap is not subject to negative-feedback control, or the genes up-regulated to control this bone formation are not those normally thought of as being involved in skeletal homeostasis. This suggested the need for a wider search for genes active during the fracture reparative process.
In this project, mRNA gene expression was measured by DNA microarray technology at various time points after fracture for young, adult, and older rats. The goal was to identify genes whose expression following fracture was altered by age. Such genes may either show reduced expression, if the age-related slowing of healing is caused by inadequate expression levels, or they may show enhanced expression, in an attempt to stimulate some poorly responding pathway. Among the genes which were differentially expressed at the fracture site with age were genes related to nerve cell activity.
In this study, we explored whether abnormal mRNA expression of genes related to nerve cell activity was associated with the slowing of skeletal repair in older rats. Abnormalities in the innervation of the fracture site will slow skeletal healing clinically [13–15] and experimentally [16–18].
Intact female Sprague-Dawley rats (Harlan Sprague-Dawley, Inc., Indianapolis, IN) were purchased at one or six months of age and housed in our vivarium in pairs until they were the proper age for experimentation. The rats were fed Teklad Rodent Diet [W] (#8604, Harlan Teklad, Madison, WI) and tap water ad libitum. The work was done in an AAALAC-accredited vivarium under protocols approved by our Institutional Animal Care and Use Committee.
Intact female Sprague-Dawley rats at 6 (young), 26 (adult) or 52 (older) weeks of age, weighing 154 ± 11 g (mean ± SD), 281 ± 25 g, and 330 ± 30 g respectively, were anaesthetized with an intraperitoneal injection of ketamine and xylazine (30 mg and 5 mg/kg body weight respectively) as described earlier [7, 11]. The left knee was shaved, scrubbed with Betadine Solution (Purdue Frederick, Stamford, CT), and draped with sterile sheets. A medial incision was made at the knee, the patella was deflected laterally and a 1.0 mm hole was drilled into the intercondylar notch. An intramedullary rod (1.0 mm diameter, stainless steel, type 304V, O-SWGX-400, Small Parts, Miami Lakes, FL) was placed retrograde into the left femur . The incision was closed with wound clips. A closed simple transverse mid-diaphyseal femoral fracture was induced with a Bonnarens and Einhorn device . Randomly selected rats from among those scheduled for surgery were used for 0 time no-fracture sham controls. Rats were euthanized at 0, 0.4, 1, 2, 4, and 6 weeks after fracture for a total of 6 time points at each of the 3 ages. Six rats per time point per age group were selected for microarray analysis (2 rats/array). Radiographs were made at fracture, at 1 week after fracture, and at euthanasia. The femora were rapidly harvested, and one third of the femoral length, centered on the fracture site, was collected. This contained the fracture callus with associated cortical bone and marrow and was frozen in liquid nitrogen and stored at -75 C.
RNA Sample Preparation and Microarray Processing
Samples were prepared as described in the Affymetrix GeneChip Expression Analysis Technical Manual (copyright 2001, Affymetrix, Inc., Santa Clara, CA, Rev. 1, Part number 701021, http://www.affymetrix.com). The sample preparation is described here in brief. Total RNA was extracted from the tissue by TRIzol (Invitrogen Life Technologies, Carlsbad, CA) with disruption of the tissue in a Brinkman Polytron homogenizer. RNA from two rats of the same age and time point was pooled for each microarray sample. Samples with 30 μg RNA were purified on RNeasy columns by Qiagen (Valencia, CA, P/N 74104) and then converted to double-stranded cDNA with a Superscript Double Stranded cDNA Synthesis Kit (Invitrogen Life Technologies, P/N 11917-010). The cDNA was then expressed as biotin-labeled cRNA by in vitro transcription (IVT) with the Enzo RNA Transcript Labeling Kit (Affymetrix, P/N 900182). Each sample was spiked with bioB, bioC, bioD, and cre (Affymetrix P/N 900299). The biotin-labeled cRNA was fragmented non-enzymatically. The fragmented cRNA was hybridized to 54 Rat U34A microarrays (Affymetrix P/N 900249) in the Affymetrix hybridization buffer for 16 hours at 45 C. The hybridized arrays were washed and stained in the Affymetrix Fluidics Station 400 to attach fluorescent labels to the biotin, followed by biotin-labeled antibody, and then a second staining with fluorescent labeling of the biotin. Each array was scanned twice by the Agilent GeneArray Scanner G2500A (Agilent Technologies, Palo Alto, CA). Three arrays from three independent samples (six rats) were done for each age at each time point.
The Rat U34A GeneChip Microarray has probe sets for over 8,700 rat genes. Most probe sets have 20 different probes for the same gene on each array with 20 additional mismatch controls. The data were analyzed with Affymetrix Microarray Suite 5.0 and Affymetrix Data Mining Tool 3.0 software. Microarray Suite was used to scale the mRNA expression (signal value) of all genes to an average of 500 for each array. For each gene, the software reported a signal value and a Present/Marginal/Absent call. This latter algorithm was a statistical comparison of the variation among the several probe sets for each gene compared to the noise level and gave a call for each gene as Present, Marginal, or Absent. The program then compared the signal value of each gene in the fractured samples against the signal value of the same gene in the unfractured control sample. The difference between the two signal levels, relative to the variability between the multiple probes for each gene, yielded a probability of change due to chance alone. Genes with p less than 0.005 were judged significantly different from the same gene in the unfractured sample. This more conservative p value was employed to minimize false positive responses.
The Data Mining Tool was used for cluster analysis with the Self Organizing Map (SOM) algorithm. The data were clustered on the signal values between 20 and 20,000 with the maximum/minimum ratio of at least 3.0 and the maximum – minimum difference of at least 100. One hundred clusters were specified.
Nerve-related genes were identified by searches for nerve-related names in the gene descriptions of each gene on the microarray. This association was confirmed by a review of the information for that gene in the NetAffx web site http://www.affymetrix.com and in the PubMed database http://www.ncbi.nlm.nih.gov/entrez/query.fcgi. GenBank accession numbers and names are shown for each gene. Each graph shows the average ± SEM of the three microarrays that were done for each time point for each age. Significant changes in gene expression were demonstrated by t test and linear regression .
This report conforms to the MIAME standards of MGED http://www.mged.org. A copy of the full microarray data set has been deposited in the NCBI Gene Expression Omnibus http://www.ncbi.nlm.nih.gov/geo/ as series GSE594.
On each array, on average, 5,200 genes were scored as absent, and 3,300 as present. Of these, 1,159 were significantly up-regulated and 928 were significantly down-regulated at two weeks after fracture in the adult rats of the first series (see Additional File 1). Up-regulated genes included cytokines and matrix genes for both cartilage and bone. Down-regulated genes included genes related to blood cell synthesis and mitochondrial function.
SOM clusters identified genes up- or down-regulated by fracture. Most genes affected by fracture followed the same time course at all three ages. These genes showed approximately the same peak expression level and regressed to baseline at about the same time point at all three ages. Among the genes affected by fracture were a number of genes associated with nerve cells. These were selected for more intense analysis.
Similar responses at all three ages
Nerve-related genes up-regulated by fracture at all three ages
Genes related to apoptosis
Cardiac sodium/calcium exchanger (Slc8a1)
Leucopus neurofibromatosis protein – type 1 (NF1)
Signal peptidase complex 18kD
Genes up-regulated by injury
Galanin (shown in Fig. 2)
Rat novel protein expressed with nerve injury (sterol-C4-methyl oxidase-like)
Genes related to nerve cell differentiation and growth
Glia maturation factor beta
Glia-derived neurite-promoting factor (serine (or cysteine) proteinase inhibitor, clade E, member 2)
Hippocampal cholinergic neurostimulating peptide (phosphatidylethanolamine binding protein)
MEGF 5, slit homolog 3
N-cadherin, Testicular (cadherin 2)
Nerve growth factor induced factor A (early growth response 1)
Pleiotrophin (heparin binding growth factor 8, Hbnf, neurite growth promoting factor 1) (Fig. 2)
Receptor-linked protein tyrosine phosphatase type D (protein tyrosine phosphatase, receptor type D)
Survival motor neuron
Synaptotagmin interacting protein zygin II (fasciculation and elongation protein zeta 2)
Neuronal olfactomedin-related ER localized protein
PSD-95/SAP 90 associated protein-4 (disks large-associated protein 4)
RAB14 (GTPase Rab 14)
RTK40 homolog (tyro 10, discordin domain receptor family, member 2)
SC65 – Synaptonemal complex protein
Taurine transporter (Slc6a6)
Vacuolar protein sorting homolog r-vps33b
Nerve-related genes down-regulated by fracture at all three ages
Genes associated with the growth cone of nerve fibers
Axonal glycoprotein TAG-1 (Contactin 2) (shown in Fig. 2)
Neuronal activity-regulated pentraxin (Narp)
Neural cell adhesion molecule L1
Genes associated with synaptic signaling pathways
GABA-A receptor delta subunit
Na+/Cl--dependent neurotransmitter transporter
Neurotransmitter transporter rB21a (X transporter protein 3)
Synaptogyrin (synaptogyrin 1)
Synaptotagmin III (synaptotagmin 3)
Synuclein 1 (synuclein alpha)
Glial cells missing protein homolog (Gcm1, glial cells missing homolog a)
Glial-derived neurotrophic factor
Neuron-specific protein PEP-19 (Purkinje cell protein 4)
Protein kinase C-regulated chloride channel
Defects in the older rats
Nerve-related genes with prolonged down-regulation after fracture in older rats
C kinase substrate calmodulin-binding protein (neurogranin) (shown in Fig. 3)
G coupled protein receptor UHR-1 (G protein coupled receptor 10)
GABA-B receptor 2 (G protein-coupled receptor 51)
Neuronatin alpha (neuronatin)
Neuropeptide Y (shown in Fig. 3)
Fatty acid coenzyme A ligase, long chain 6
RoBo-1 (Slc11a1) (shown in Fig. 3)
Sensory neuron synuclein (gamma-synuclein)
Nerve-related genes with a greater up-regulation in younger rats
Genes related to nerve cell differentiation and the cell cycle
Neuron-derived orphan receptor-2
PCTAIRE 3 (shown in Fig. 4)
Protein tyrosine phosphatase, receptor-type, Z polypeptide 1 (shown in Fig. 4)
Genes related to the synapse and sensory perception
Proton gated cation channel DRASIC
Synaptic scaffolding molecule (S-SCAM, activin receptor interacting protein 1) (shown in Fig. 4)
Sodium-dependent neurotransmitter transporter
Nerve-related genes with a greater up-regulation in older rats
Genes related to signals and signal transduction
Acetylcholine receptor alpha subunit (cholinergic receptor, nicotinic, alpha polypeptide 1)
Brain type II calcium/calmodulin dependent protein kinase beta subunit
Genes related to cell adhesion
F-spondin (shown in Fig. 5)
Neurocan (chondroitin sulfate proteoglycan 3)
Non-processed neurexin 1-beta (neurexin 1)
TSP-4 (thrombospondin-4) (shown in Fig. 5)
Activity and neurotransmitter-induced early gene 4 (ania-4) (shown in Fig. 5)
Serine protease (complement component 1, s subcomponent)
For each gene for each array, the Microarray Suite software reported a statistical decision as to whether the mRNA was Present, Marginal, or Absent. We have reviewed these calls for the genes shown in Figures 2,3,4,5. For Figure 2, the Present/Marginal/Absent calls were: Fig. 2 Top: 53/1/0; Fig. 2 Middle: 39/2/13; and Fig. 2 Bottom: 0/0/54. For Figure 3, the calls were: Fig. 3 Top: 45/2/7; Fig. 3 Middle: 7/0/47; and Fig. 3 Bottom: 32/6/16. For Figure 4, the calls were: Fig. 4 Top: 0/0/54; Fig. 4 Middle: 0/0/54; and Fig. 4 Bottom: 51/0/3. For Figure 5, the calls were: Fig. 5 Top: 45/1/8; Fig. 5 Middle: 52/0/2; and Fig. 5 Bottom: 54/0/0.
In this study, as in our earlier work [7, 10–12], the time required to reach radiographic union after femoral fracture increased with age in the female rat. This slowing of fracture repair with age is associated with changes in the mRNA expression of specific genes within the healing fracture site [10–12]. To study this further, microarray technology was used to identify additional genes whose mRNA expression was affected by skeletal fracture.
More than two-thirds of the detectable genes on the rat U34A microarray have a change in mRNA expression level following fracture . Most of these genes were not known to participate in the healing process of bone before the advent of microarray technology [21, 22]. This reflects changes in both the types of cells at the fracture site as well as changes in the activity of the existing cells. Among the cells affected by fracture are nerve fibers. Protein and mRNA of genes related to neuronal functioning are found in intact bone and in the fracture callus [23–29]. Since proper innervation of the fracture site is needed for fracture repair clinically [13–15] and experimentally [16–18], this led to the hypothesis that the age-related slowing of fracture repair may be related to the abnormal nerve cell activity at the fracture site.
To evaluate this hypothesis, nerve-related genes were studied from among the genes present on the Affymetrix Rat U34A microarray. Genes were identified for which the mRNA response to femoral fracture was changed in the older rats compared to the young rats. Three types of change with age were found:
1. The mRNA expression levels of the genes shown in Table 3 and Figure 3 were decreased by fracture. While gene expression in the young rats was approaching pre-fracture levels by six weeks after fracture, gene expression showed minimal return to normal in older rats. Genes in this category were all related to signaling molecules or to signal receptors (references shown in Table 3).
2. Other nerve-related genes had strong up-regulation after fracture in young rats but only mild up-regulation in older rats. These are shown in Table 4 and Figure 4. This partial loss of function with age was observed in genes associated with nerve cell differentiation or cell cycle or genes related to synaptic structure (references cited in Table 4).
3. A third set of genes was increased in mRNA expression by fracture, but the increase was greater in the older rats. These are shown in Table 5 and Figure 5. Many of these genes were related to cell adhesion or to cell signal or signal transduction (references cited in Table 5).
All three classes of genes showed altered expression in the older rats compared to young rats. We hypothesize that bone fracture may physically disrupt nerve fibers in bone. A sub-population of these skeletal nerve fibers may regrow into the fracture site or regain function at a slower rate in older rats. This may account for the failure to recover from low mRNA values for the first group (prolonged down-regulation) or the failure to up-regulate mRNA expression adequately after fracture in the older rats in the second group (diminished up-regulation). Other genes in the third group with increased levels of mRNA after fracture in the older rats may represent attempts to stimulate nerve regrowth or other processes that are not responding. This may represent negative-feedback-induced up-regulation caused by effector cell resistance. Taken together, these changes in nerve cell function with age may contribute to the slowing of fracture repair in older rats. It must be pointed out that the associations noted here do not necessarily reflect cause and effect. It is also possible that the delayed re-innervation of the fracture site is an effect of the delayed healing in the older rats and not a cause of the delayed healing.
Experimental studies have been done to detect the role of innervation on fracture healing. Studies of sectioning the sciatic nerve in concert with tibial fracture have been reported to speed fracture healing [30–33]. However, sectioning both femoral and sciatic nerves inhibits fracture healing . Aro et al.  have reported mechanoreceptors (Pacinian corpuscles) in the periostium of the rat fibula, which, if removed, lead to non-union . Direct application of nerve growth factor to the fracture site increases healing in the rat rib .
In humans, abnormal bone healing is also associated with lack of nerve activity at the fracture site. Nagano et al.  have noted scaphoid nonunion in the wrists of patients with neuroarthropathy from a long-standing nerve palsy. Santavirta et al.  have found a lack of peripheral innervation at the fracture site of noninfected fractures with delayed union or nonunion of diaphyseal bones. Nordstrom et al.  have found a lack of stromal innervation associated with delayed union or pseudoarthrosis in spondylolysis.
Humans [1–6] show a slowing of fracture healing with increasing age as do rats [7–9]. The cause of the slowing of fracture healing with age is not well understood. The femora of young rats regain normal biomechanical properties by 4 weeks after fracture, while adults take 12 weeks, and older rats require in excess of 6 months . This model presents an opportunity to elucidate novel genes important to this healing process. The slowing could reflect a loss of function as some processes essential for the rapid healing of fractures in young animals are inhibited with age. Alternatively, the slowing of skeletal repair with age may be caused by partial resistance of the healing process to stimulation in adult or older individuals. Such resistance should result in enhanced stimulation by regulatory systems to attempt to evoke a healing response. Both patterns were seen among the genes studied in this report. These genes are candidates for further study.
These changes with age are not limited to genes related to neuronal activity. We have also noted similar changes in genes related to mitochondrial activity . It is likely that the age-related changes in fracture repair are caused by failure of several metabolic pathways. Methods, such as DNA microarrays, which sample many different biological pathways will be useful in defining these novel, multi-faceted defects.
The specificity of these changes is seen in the majority of the nerve-related genes for which the expression pattern following fracture was unaffected by age. These transcripts had similar increases or decreases following fracture in the young, adult, and older rats. These uniform responses suggest that most metabolic patterns were unaffected by age. Nerve-related genes similarly up-regulated by femoral fracture at all three ages were broadly related to differentiation and growth of nerve cells, to known up-regulation following nerve injury, or to association with apoptosis (references cited in Table 1). Some of these genes were slower to return to baseline values in older rats, such as galanin and TAG-1. In contrast, nerve-related genes similarly down-regulated by femoral fracture at all three ages were broadly related to the nerve growth cone or to synaptic signaling pathways (references cited in Table 2).
In this study gene expression was measured by quantification of the mRNA level for each gene with microarray technology. It must be kept in mind that there are other control systems which influence the protein synthetic rate and also protein degradation. Protein synthesis will be low in the absence of mRNA for that gene, but elevated mRNA levels are not a guarantee that protein levels will also be elevated for that gene. Changes noted at the mRNA level will need to be confirmed at the protein and structural levels. Assignment of the genes studied herein as nerve-related is made on the basis of currently available information. Other cell types in the fracture callus may also express these genes. Histological studies will permit the association of these genes with specific cell types within the fracture callus. These experiments are now in progress.
We have compared mRNA gene expression by microarray to that measured by reverse transcription – polymerase chain reaction (RT-PCR). Good correlation was found between the two methods if the transcripts were judged mostly present, the signal level did not approach the upper limit of the detector, and the probe sets or PCR primers were from the same region of the gene . Some other genes, even though most samples were judged absent, also gave good correlation between the two methods. These latter genes were at the upper range of the absent calls and had good precision between samples . The genes reported herein have the marked variation in mRNA levels that have been reported previously in fracture samples [10, 11] with large changes in expression after fracture which return to the prefracture levels as healing progresses. The finding here of moderate signal levels, good precision among the three samples for each time point at each age, and a strong response to fracture indicate the ability of this technology to report changes in mRNA levels for these genes.
In summary, most genes respond to bone fracture with altered mRNA gene expression, including genes related to neuronal functioning. However, a number of these genes responded to fracture differently in older rats than in young rats. Such differential expression with age may reflect altered cell functioning at the fracture site that may be related to the slowing of fracture healing in older rats.
This study was supported in part by grants from the North Carolina Biotechnology Center and the Orthopaedic Trauma Association. We thank Drs. Scott Porter, James Fiechtl and Daniel Heiner for their surgical assistance. We thank Ms. Virginia Mooney, Mr. Michael Steuerwald, and Ms. Jessica Heath for their technical assistance and Ms. Carolyn Ayers for her secretarial assistance. A preliminary report of this study has appeared .
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